Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus

Polyakov, Dmitry; Sinitsyna, Ekaterina; Grudinina, Natalia; Antipchik, Mariia; Sakhabeev, Rodion; Korzhikov-Vlakh, Viktor; Shavlovsky, Mikhail; Korzhikova-Vlakh, Evgenia; Tennikova, Tatiana

Hepatitis C is one of the most common social diseases in the world. The improvements in both the early diagnostics of the hepatitis C and the treatment of acute viremia caused by hepatitis C virus are undoubtedly an urgent task. In present work, we offered the micro- and nanotraps for the capturing of HCV. As a capturing moiety, we designed and synthesized in E. coli a fusion protein consisting of large extracellular loop of CD81 receptor and streptavidin as spacing part. The obtained protein has been immobilized on the surface of PLA-based micro- and nanoparticles. The developed trapping systems were characterized in terms of their physico-chemical properties. In order to illustrate the ability of developed micro- and nanotraps to bind HCV, E2 core protein of HCV was synthesized as a fusion protein with GFP. Interaction of E2 protein and hepatitis C virus-mimicking particles with the developed trapping systems were testified by several methods.