The natural history and transmission potential of asymptomatic SARS-CoV-2 infection

Chau, Nguyen Van Vinh; Thanh Lam, Vo; Thanh Dung, Nguyen; Yen, Lam Minh; Minh, Ngo Ngoc Quang; Hung, Le Manh; Ngoc, Nghiem My; Dung, Nguyen Tri; Man, Dinh Nguyen Huy; Nguyet, Lam Anh; Nhat, Le Thanh Hoang; Nhu, Le Nguyen Truc; Ny, Nguyen Thi Han; Hong, Nguyen Thi Thu; Kestelyn, Evelyne; Dung, Nguyen Thi Phuong; Xuan, Tran Chanh; Hien, Tran Tinh; Thanh Phong, Nguyen; Tu, Tran Nguyen Hoang; Geskus, Ronald B; Thanh, Tran Tan; Thanh Truong, Nguyen; Binh, Nguyen Tan; Thuong, Tang Chi; Thwaites, Guy; Tan, Le Van

BACKGROUND: Little is known about the natural history of asymptomatic SARS-CoV-2 infection or its contribution to infection transmission. METHODS: We conducted a prospective study at a quarantine center for COVID-19 in Ho Chi Minh City, Vietnam. We enrolled quarantined people with RT-PCR-confirmed SARS-CoV-2 infection, collecting clinical data, travel and contact history, and saliva at enrolment and daily nasopharyngeal throat swabs (NTS) for RT-PCR testing. We compared the natural history and transmission potential of asymptomatic and symptomatic individuals. RESULTS: Between March 10(th) and April 4(th), 2020, 14,000 quarantined people were tested for SARS-CoV-2; 49 were positive. Of these, 30 participated in the study: 13(43%) never had symptoms and 17(57%) were symptomatic. 17(57%) participants acquired their infection outside Vietnam. Compared with symptomatic individuals, asymptomatic people were less likely to have detectable SARS-CoV-2 in NTS samples collected at enrolment (8/13 (62%) vs. 17/17 (100%) P=0.02). SARS-CoV-2 RNA was detected in 20/27 (74%) available saliva; 7/11 (64%) in the asymptomatic and 13/16 (81%) in the symptomatic group (P=0.56). Analysis of the probability of RT-PCR positivity showed asymptomatic participants had faster viral clearance than symptomatic participants (P<0.001 for difference over first 19 days). This difference was most pronounced during the first week of follow-up. Two of the asymptomatic individuals appeared to transmit the infection to up to four contacts. CONCLUSIONS: Asymptomatic SARS-CoV-2 infection is common and can be detected by analysis of saliva or NTS. NTS viral loads fall faster in asymptomatic individuals, but they appear able to transmit the virus to others.