Molecular detection and genotyping of bovine viral diarrhea virus in Western China

Chang, Lingling; Qi, Yanping; Liu, Dan; Du, Qian; Zhao, Xiaomin; Tong, Dewen

BACKGROUND: Bovine viral diarrhea virus (BVDV) is an important global viral pathogen of cattle and other ruminants. To survey the infection rate and genetic diversity of BVDV in western China, a total of 1234 serum samples from 17 herds of dairy cattle, beef cattle and yak in 4 provinces were collected in 2019. RESULTS: All the 1234 serum samples were screened individually for BVDV by RT-PCR. Our results demonstrated that the average positive rate of BVDV was 7.2% (89/1234) in animals and 82.4% (14/17) in herds. Thirteen BVDV strains were isolated from RT-PCR positive clinical samples and they were all NCP biotype. BVDV-1a and 1c subgenotypes were identified from 22 selected virus isolates in 14 BVDV-positive herds. These results confirmed that BVDV-1a and BVDV-1c were circulating in western China, similar to the BVDV epidemics in cattle in other regions of China. CONCLUSIONS: This study provides data for monitoring and vaccination strategies of BVDV in western China. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-021-02747-7.