Evaluating an Upper Respiratory Disease Panel on the Portable MinION Sequencer

Wanda J. Lyon; Zachary K. Smith; Brian Grier; James Baldwin; Clarise R. Starr

The discrepancies observed between whole genome followed with RT-PCR and amplicon sequencing were minimal in regards to identity alignment (Tables 2 and 3 ). The base-calling analysis for the MinION nanopore sequencer is an area of active development, as well as flow cell configurations, protocols for kits, and associated kit chemistries. The best quality sequence data are termed 2D reads, while 1D reads are from the template strand. Although the accuracy of MinION sequence data is an area of active development, with recent development of a new nanopore for the flow cells, one should expect increased data quality with each increment in flow cell configuration. Data quality has improved and was demonstrated in this study with each improvement in kit chemistry and flow cell type. This study shows that the platform can be All rights reserved. No reuse allowed without permission.