Author: Vanaerschot, Manu; Mann, Sabrina A.; Webber, James T.; Kamm, Jack; Bell, Sidney M.; Bell, John; Hong, Si Noon; Nguyen, Minh Phuong; Chan, Lienna Y.; Bhatt, Karan D.; Tan, Michelle; Detweiler, Angela M.; Espinosa, Alex; Wu, Wesley; Batson, Joshua; Dynerman, David; Wadford, Debra A.; Puschnik, Andreas S.; Neff, Norma; Ahyong, Vida; Miller, Steve; Ayscue, Patrick; Tato, Cristina M.; Paul, Simon; Kistler, Amy; DeRisi, Joseph L.; Crawford, Emily D.
Title: Identification of a polymorphism in the N gene of SARS-CoV-2 that adversely impacts detection by a widely-used RT-PCR assay Cord-id: eagq57d5 Document date: 2020_8_26
ID: eagq57d5
Snippet: We identify a mutation in the N gene of SARS-CoV-2 that adversely affects annealing of a commonly used RT-PCR primer; epidemiologic evidence suggests the virus retains pathogenicity and competence for spread. This reinforces the importance of using multiple targets, preferably in at least 2 genes, for robust SARS-CoV-2 detection. Article Summary Line A SARS-CoV-2 variant that occurs worldwide and has spread in California significantly affects diagnostic sensitivity of an N gene assay, highlighti
Document: We identify a mutation in the N gene of SARS-CoV-2 that adversely affects annealing of a commonly used RT-PCR primer; epidemiologic evidence suggests the virus retains pathogenicity and competence for spread. This reinforces the importance of using multiple targets, preferably in at least 2 genes, for robust SARS-CoV-2 detection. Article Summary Line A SARS-CoV-2 variant that occurs worldwide and has spread in California significantly affects diagnostic sensitivity of an N gene assay, highlighting the need to employ multiple viral targets for detection.
Search related documents:
Co phrase search for related documents- Try single phrases listed below for: 1
Co phrase search for related documents, hyperlinks ordered by date