Selected article for: "amplify dna and assay amplify dna"

Author: Benson, Robert; Tondella, Maria L; Bhatnagar, Julu; Carvalho, Maria da Glória S; Sampson, Jacquelyn S; Talkington, Deborah F; Whitney, Anne M; Mothershed, Elizabeth; McGee, Lesley; Carlone, George; McClee, Vondguraus; Guarner, Jeannette; Zaki, Sherif; Dejsiri, Surang; Cronin, K; Han, Jian; Fields, Barry S
Title: Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens.
  • Cord-id: z73pxely
  • Document date: 2008_1_1
  • ID: z73pxely
    Snippet: The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.
    Document: The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.

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