Selected article for: "amplicon sequencing and identity alignment"
Author: Wanda J. Lyon; Zachary K. Smith; Brian Grier; James Baldwin; Clarise R. Starr
Title: Evaluating an Upper Respiratory Disease Panel on the Portable MinION Sequencer
  • Document date: 2018_10_5
    • ID: 3begdfx2_31
    Snippet: Sequencing of URD viruses and bacteria was accomplished on the MinION nanopore sequencer using both whole genome and amplicon sequencing. Having the ability to sequence amplicons and whole genomes in a mobile, small-footprint platform is attractive when collecting and analyzing samples in the field, and these qualities are also desirable as sequencing methods move toward environmental and surveillance applications. These methods, data, and result.....
    Document: Sequencing of URD viruses and bacteria was accomplished on the MinION nanopore sequencer using both whole genome and amplicon sequencing. Having the ability to sequence amplicons and whole genomes in a mobile, small-footprint platform is attractive when collecting and analyzing samples in the field, and these qualities are also desirable as sequencing methods move toward environmental and surveillance applications. These methods, data, and results represent a practical and novel application for utilizing MinION nanopore sequencing technology. The data in this study were generated with both R7.3 and 9.4 chemistry flow cells, which accurately identified a panel of URD organisms. As the MinION technology improves, sequencing will generally become cheaper, faster, and more accurate as demonstrated in this study with improvements in identity alignment in the >92% range (Tables 2 and 3 ) as compared to earlier experiments that were in the 30% range [38] .

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