Author: Wanda J. Lyon; Zachary K. Smith; Brian Grier; James Baldwin; Clarise R. Starr
Title: Evaluating an Upper Respiratory Disease Panel on the Portable MinION Sequencer Document date: 2018_10_5
ID: 3begdfx2_23
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/436600 doi: bioRxiv preprint There was minimal sequencing bias seen within the barcoded library pool (data not shown); however, the number of reads were somewhat different among the tested organisms and probably due to an error in quantification of the input library prior to pooling. Future barcoding pooling protocols should includ.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/436600 doi: bioRxiv preprint There was minimal sequencing bias seen within the barcoded library pool (data not shown); however, the number of reads were somewhat different among the tested organisms and probably due to an error in quantification of the input library prior to pooling. Future barcoding pooling protocols should include a Tapestation (Agilent) quantification in terms of fragment length, which can be used to calculate of the nM concentration; nevertheless, this experiment demonstrated that the use of Qubit quantifications is sufficient for rapid pooling of multiplex libraries.
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