Author: Wanda J. Lyon; Zachary K. Smith; Brian Grier; James Baldwin; Clarise R. Starr
Title: Evaluating an Upper Respiratory Disease Panel on the Portable MinION Sequencer Document date: 2018_10_5
ID: 3begdfx2_33
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/436600 doi: bioRxiv preprint applied for rapid microbial detection with 3-hour run time, which provides sufficient data to generate a reliable identification. As sequencing yield, quality, and turnaround times continue to improve, it is anticipated that nanopore sequencing will challenge mainstays such as PCR for identification of .....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/436600 doi: bioRxiv preprint applied for rapid microbial detection with 3-hour run time, which provides sufficient data to generate a reliable identification. As sequencing yield, quality, and turnaround times continue to improve, it is anticipated that nanopore sequencing will challenge mainstays such as PCR for identification of unknown samples. Amplicon and rapid WGS was successfully utilized as an approach for pathogen detection. Low titer viral nasal wash samples were was positively identified after WGA even when samples had high human read counts due to a carry-over human NA contribution. Parainfluenza 1, Parainfluenza 2, Parainfluenza 3 and adenovirus nasal wash samples had 81%, 90%, 95% and 81% of the read count aligning to human references genomes, respectively.
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