Author: Voon, Kenny; Johari, Nur Alia; Lim, Khai Lone; Wong, Siew Tung; Khaw, Loke Tim; Wong, Shew Fung; Chan, Elaine W L; Chan, Kok Keong; Tan, Boon Keat; Ramzi, Nurul Hanis; Lim, Patricia K C; Sulaiman, Lokman H
Title: COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR. Cord-id: f3d5ywz3 Document date: 2021_5_5
ID: f3d5ywz3
Snippet: The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume less reagents by increasing the capacity of testing and reducing the number of experiments performed, and are therefore especially suitable for under-d
Document: The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume less reagents by increasing the capacity of testing and reducing the number of experiments performed, and are therefore especially suitable for under-developed countries with limited resources. Here, we propose a simple, reliable pooling strategy for COVID-19 testing using clinical nasopharyngeal (NP) and/or oropharyngeal (OP) swabs. The strategy includes the pooling of 10 NP/OP swabs for extraction and subsequent testing via quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR), and may also be applied to the screening of other pathogens.
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