Selected article for: "house real time pcr and real time pcr"
Author: Kenmoe, Sebastien; Tcharnenwa, Clarisse; Monamele, Gwladys C.; Kengne, Cyprien Nde; Ripa, Mohamadou Njankouo; Whitaker, Brett; Alroy, Karen A.; Balajee, S. Arunmozhi; Njouom, Richard
Title: Comparison of FTD® respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon
  • Cord-id: 8msm5n3b
  • Document date: 2019_1_16
    • ID: 8msm5n3b
    Snippet: Introduction: This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD®) Respiratory Pathogens 33 multiplex test. Methods: A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assa
    Document: Introduction: This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD®) Respiratory Pathogens 33 multiplex test. Methods: A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses. Results: The results showed that 179/217 (82.5%) samples were positive with the singleplex method and 183/217 (84.3%) with the FTD® Respiratory Pathogens 33 multiplex test. Excellent or satisfactory agreement was obtained for all viruses (k > 0.6) except Parainfluenzavirus 4 (k = 0.24) and influenza B (k = 0.51). Conclusion: Although the multiplex FTD kits were more expensive than the singleplex assay, the FTD kits yielded rapid results in a shorter timeframe, increasing efficiency of diagnosis.

    Search related documents:
    Co phrase search for related documents
    • acid amplification and low number: 1, 2, 3
    • acid amplification and low prevalence: 1, 2, 3, 4, 5
    • acid amplification and low respiratory: 1, 2, 3, 4
    • acid amplification and low sensitivity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
    • acid amplification and luminex molecular diagnostics: 1, 2
    • acute age respiratory infection and low prevalence: 1, 2
    • acute age respiratory infection and low respiratory: 1
    • acute age respiratory infection and low respiratory tract: 1
    • acute respiratory infection and additional study: 1, 2, 3, 4, 5, 6, 7
    • acute respiratory infection and low agreement: 1
    • acute respiratory infection and low number: 1, 2, 3, 4, 5, 6, 7, 8
    • acute respiratory infection and low prevalence: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
    • acute respiratory infection and low respiratory: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • acute respiratory infection and low respiratory tract: 1, 2, 3, 4, 5
    • acute respiratory infection and low sensitivity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
    • acute respiratory infection and luminex nxtag: 1
    • acute respiratory infection and luminex nxtag respiratory: 1
    • acute respiratory infection and luminex nxtag respiratory pathogen panel: 1
    • additional study and low number: 1, 2, 3