Author: Wanbing Liu; Lei Liu; Guomei Kou; Yaqiong Zheng; Yinjuan Ding; Wenxu Ni; Qiongshu Wang; Li Tan; Wanlei Wu; Shi Tang; Zhou Xiong; Shangen Zheng
Title: Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2 Document date: 2020_3_20
ID: lm9pay2b_7
Snippet: For IgG detection, ELISA plates were coated with rN protein. Serum sample (5 μL) 125 diluted in 100 μL dilution buffer was added to the plates. After incubation and 126 washing, HRP-conjugated monoclonal mouse anti-human IgG antibody was added to 127 the plates for detection. The other operation steps were performed as described above 128 for IgM detection. The cutoff value was calculated by summing 0.130 and the average 129 A450 of negative co.....
Document: For IgG detection, ELISA plates were coated with rN protein. Serum sample (5 μL) 125 diluted in 100 μL dilution buffer was added to the plates. After incubation and 126 washing, HRP-conjugated monoclonal mouse anti-human IgG antibody was added to 127 the plates for detection. The other operation steps were performed as described above 128 for IgM detection. The cutoff value was calculated by summing 0.130 and the average 129 A450 of negative control replicates. When A450 was below the cutoff value, the test was 130 considered negative, and when A450 was greater than or equal to the cutoff value, the 131 test was considered positive.
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