Author: Nasa Sinnott-Armstrong; Daniel Klein; Brendan Hickey
Title: Evaluation of Group Testing for SARS-CoV-2 RNA Document date: 2020_3_30
ID: bgm3bt78_36
Snippet: 1. Multi-channel pipettes enable simultaneous multi-sample manipulation by hand. Given the gridded nature of our testing system, multi-channel pipettes are likely effective. Multi-channel pipetting into a deepwell plate for pooled sample storage is likely the simplest option. For example, into a 2.5 mL deepwell plate, pipette 150 µL of each pre-extraction row and column sample to yield a total of 1.8 mL of pool for rows and 1.2mL of pool for col.....
Document: 1. Multi-channel pipettes enable simultaneous multi-sample manipulation by hand. Given the gridded nature of our testing system, multi-channel pipettes are likely effective. Multi-channel pipetting into a deepwell plate for pooled sample storage is likely the simplest option. For example, into a 2.5 mL deepwell plate, pipette 150 µL of each pre-extraction row and column sample to yield a total of 1.8 mL of pool for rows and 1.2mL of pool for columns. Then mix thoroughly and extract from standard volumes. We note that this mixing increases risk of operator exposure, so strict biosafety precautions must be adhered to.
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