Evolutionary proteomics uncovers ciliary signaling components

Monika Abedin Sigg; Tabea Menchen; Jeffery Johnson; Chanjae Lee; Semil P. Choksi; Galo Garcia; Henriette Busengdal; Gerard Dougherty; Petra Pennekamp; Claudius Werner; Fabian Rentzsch; Nevan Krogan; John B. Wallingford; Heymut Omran; Jeremy F. Reiter

To trace the ancestry of known ciliary signaling pathways, we also examined whether 303 previously identified components of these pathways were present in the sea urchin, sea anemone 304 and choanoflagellate ciliomes. Choanoflagellate genomes do not contain genes for most Hh 305 pathway components, suggesting that they do not use Hh signaling for intercellular 306 communication (King et al., 2008) . Analysis of whether ciliary Hh pathway components were 307 present in the sea urchin and sea anemone ciliomes revealed that, although canonical Hh IQ domain-containing protein E (IQCE) and EF-hand calcium binding domain-containing 319 protein 7 (EFCAB7), regulate Hh signaling by anchoring EVC and EVC2 to the proximal cilium 320 (Pusapati et al., 2014) . IQCE was present in the sea urchin ciliome and EFCAB7 was present in 321 the sea anemone ciliome. 322