Author: Monika Abedin Sigg; Tabea Menchen; Jeffery Johnson; Chanjae Lee; Semil P. Choksi; Galo Garcia; Henriette Busengdal; Gerard Dougherty; Petra Pennekamp; Claudius Werner; Fabian Rentzsch; Nevan Krogan; John B. Wallingford; Heymut Omran; Jeremy F. Reiter
Title: Evolutionary proteomics uncovers ciliary signaling components Document date: 2017_6_22
ID: 9y8r277c_100
Snippet: To visualize Actin and TUB ac , N. vectensis embryos were fixed for 1.5 minutes in 3.7% 888 Formaldehyde, 0.25% Gluteraldehyde in 33% artificial seawater and then in 3.7% 889 Formaldehyde, 0.1% Tween-20 for 1 hour at 4°C. Embryos were blocked in PBST containing 890 10% donkey serum for 1 hour and subsequently incubated with primary antibodies and Alexa 891.....
Document: To visualize Actin and TUB ac , N. vectensis embryos were fixed for 1.5 minutes in 3.7% 888 Formaldehyde, 0.25% Gluteraldehyde in 33% artificial seawater and then in 3.7% 889 Formaldehyde, 0.1% Tween-20 for 1 hour at 4°C. Embryos were blocked in PBST containing 890 10% donkey serum for 1 hour and subsequently incubated with primary antibodies and Alexa 891
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