Selected article for: "cDNA synthesis kit and real time"
Author: Monika Abedin Sigg; Tabea Menchen; Jeffery Johnson; Chanjae Lee; Semil P. Choksi; Galo Garcia; Henriette Busengdal; Gerard Dougherty; Petra Pennekamp; Claudius Werner; Fabian Rentzsch; Nevan Krogan; John B. Wallingford; Heymut Omran; Jeremy F. Reiter
Title: Evolutionary proteomics uncovers ciliary signaling components
  • Document date: 2017_6_22
    • ID: 9y8r277c_109
    Snippet: for 10 minutes and immunostained as described for mammalian cells. Germany) and cDNA was synthesized using iScript cDNA Synthesis Kit (BioRad). Quantitative 999 PCR was performed using EXPRESS SYBR GreenER with Premixed ROX (Invitrogen) and the 1000 7900HT Fast Real-Time PCR System (ThermoFisher Scientific). Relative expression was 1001 calculated using the delta-delta CT method (Livak and Schmittgen, 2001) . For measuring 1002 All rights reserve.....
    Document: for 10 minutes and immunostained as described for mammalian cells. Germany) and cDNA was synthesized using iScript cDNA Synthesis Kit (BioRad). Quantitative 999 PCR was performed using EXPRESS SYBR GreenER with Premixed ROX (Invitrogen) and the 1000 7900HT Fast Real-Time PCR System (ThermoFisher Scientific). Relative expression was 1001 calculated using the delta-delta CT method (Livak and Schmittgen, 2001) . For measuring 1002 All rights reserved. No reuse allowed without permission.

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