Author: Monika Abedin Sigg; Tabea Menchen; Jeffery Johnson; Chanjae Lee; Semil P. Choksi; Galo Garcia; Henriette Busengdal; Gerard Dougherty; Petra Pennekamp; Claudius Werner; Fabian Rentzsch; Nevan Krogan; John B. Wallingford; Heymut Omran; Jeremy F. Reiter
Title: Evolutionary proteomics uncovers ciliary signaling components Document date: 2017_6_22
ID: 9y8r277c_110
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/153437 doi: bioRxiv preprint expression of Enkur, the geometric mean of Ubiquitin C (Ubc) and Hydroxymethylbilane 1003 synthase (Hmbs) expression was used as the endogenous control for normalization. See Table S9 1004 for primer sequences. The data were analyzed using Microsoft Excel and plotted using Prism7. To generate the X. la.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/153437 doi: bioRxiv preprint expression of Enkur, the geometric mean of Ubiquitin C (Ubc) and Hydroxymethylbilane 1003 synthase (Hmbs) expression was used as the endogenous control for normalization. See Table S9 1004 for primer sequences. The data were analyzed using Microsoft Excel and plotted using Prism7. To generate the X. laevis ENKUR-GFP expression construct, full length of Enkurin 1017 cDNA was amplified from X. laevis RNA and cloned into pCS10R vector fused with N-terminal 1018 GFP using SalI and NotI. To generate the X. laevis in situ probe, the above plasmid was cut with 1019 SalI and synthesized using the T7 promoter. Pitx2c in situ probe, membrane-RFP (CAAX motif 1020 fused to RFP) and Centrin 4-BFP plasmids described in (Toriyama et al., 2016) . 1021
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