Evolved sequence features within the intrinsically disordered tail influence FtsZ assembly and bacterial cell division

Megan C. Cohan; Ammon E. Posey; Steven J. Grigsby; Anuradha Mittal; Alex S. Holehouse; Paul J. Buske; Petra A. Levin; Rohit V. Pappu

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/301622 doi: bioRxiv preprint segregated within the linear sequence. The screening of intra-chain repulsions by attractions leads to expanded conformations low κ sequences. As κ increases, attractions between oppositely charged blocks induce chain compaction. (C) Normalized conservation scores for different regions of FtsZ. The conservation scores, in terms of percent sequence identities, were computed using CLUSTAL-W. In each box and whisker plot, the solid horizontal line represents the mean conservation score. The box outlines the middle 50% of the values, and the whiskers represent the entire distribution. All conservation scores were normalized to the scores for sequences of G-actin. By definition, the mean normalized score is one for G-actin. The mean normalized conservation scores are ~0.57 and ~0.55 for the core domain and CTP motifs, respectively. This is to be contrasted with the low scores for the CTL and CTV sequences. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/301622 doi: bioRxiv preprint mean R g values for each variant by the square root of the length n of the corresponding sequence. The extra residues present in each CTL variant were a product of cloning. These data show that the length normalized R g is high for CTT sequences with κ values that are low. In contrast, we observe a monotonic decrease as κ values increase beyond that of the WT. (D) Two-dimensional conformational distributions from atomistic simulations of CTT sequences for the WT. These distributions quantify the joint probability density p(R g ,δ * ) of sizes (R g ) and