Author: Megan C. Cohan; Ammon E. Posey; Steven J. Grigsby; Anuradha Mittal; Alex S. Holehouse; Paul J. Buske; Petra A. Levin; Rohit V. Pappu
Title: Evolved sequence features within the intrinsically disordered tail influence FtsZ assembly and bacterial cell division Document date: 2018_4_14
ID: 2rzfuy33_47
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/301622 doi: bioRxiv preprint asked if these variants promote or hinder FtsZ polymerization in vitro. We performed light scattering assays for CTLV5 and CTLV6 in the presence and absence of GTP. The light scattering data obtained in the presence of GTP show a WT-like decrease in light scattering intensity at physiological concentrat.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/301622 doi: bioRxiv preprint asked if these variants promote or hinder FtsZ polymerization in vitro. We performed light scattering assays for CTLV5 and CTLV6 in the presence and absence of GTP. The light scattering data obtained in the presence of GTP show a WT-like decrease in light scattering intensity at physiological concentrations of monovalent salt ( Figure 5A ). In 50 mM KCl, CTLV5
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