Author: Nicola F Fletcher; Luke W Meredith; Emma L Tidswell; Steven R Bryden; Daniel Gonçalves-Carneiro; Yasmin Chaudhry; Claire Shannon-Lowe; Michael A Folan; Daniella A Lefteri; Marieke Pingen; Dalan Bailey; Clive S McKimmie; Alan W Baird
Title: A novel antiviral formulation inhibits a range of enveloped viruses. Document date: 2020_3_30
ID: nly9vojr_42
Snippet: Orf (MRI-scab) was harvested from supernatants of primary cultures of foetal lamb skin (FLS) cells, filtered through a 0.45μm filter and then centrifuged for 12 hours at 12,000rpm in a bench-top centrifuge (Eppendorf). Virus was placed on formvar coated copper 400 electron microscopy grids for 5 minutes; excess supernatant was removed and the grids were stained with 1% uranyl acetate. Grids were visualized using a Tecnai 12 transmission electron.....
Document: Orf (MRI-scab) was harvested from supernatants of primary cultures of foetal lamb skin (FLS) cells, filtered through a 0.45μm filter and then centrifuged for 12 hours at 12,000rpm in a bench-top centrifuge (Eppendorf). Virus was placed on formvar coated copper 400 electron microscopy grids for 5 minutes; excess supernatant was removed and the grids were stained with 1% uranyl acetate. Grids were visualized using a Tecnai 12 transmission electron microscope.
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