Author: Pingping Zhang; Qi Gao; Tang Wang; Yuehua Ke; Fei Mo; Ruizhong Jia; Wanbing Liu; Lei Liu; Shangen Zheng; Yuzhen Liu; Luping Li; Yao Wang; Lei Xu; Kun Hao; Ruifu Yang; Shiyue Li; Changqing Lin; Yong Zhao
Title: Evaluation of recombinant nucleocapsid and spike proteins for serological diagnosis of novel coronavirus disease 2019 (COVID-19) Document date: 2020_3_20
ID: 7atoko6c_5
Snippet: Recently, S-RBD from HCoV-19 was reported to have higher affinity than 10 SARS-CoV to angiotensin-converting enzyme 2, and also to lack cross-reactivity with 11 monoclonal antibodies against S-RBD from SARS-CoV [3], highlighting the potential 12 value of the S protein for diagnosing HCoV-19. As a transmembrane protein with 13 hydrophobic regions, the S protein is better prepared using a eukaryotic expression 14 system. 15.....
Document: Recently, S-RBD from HCoV-19 was reported to have higher affinity than 10 SARS-CoV to angiotensin-converting enzyme 2, and also to lack cross-reactivity with 11 monoclonal antibodies against S-RBD from SARS-CoV [3], highlighting the potential 12 value of the S protein for diagnosing HCoV-19. As a transmembrane protein with 13 hydrophobic regions, the S protein is better prepared using a eukaryotic expression 14 system. 15
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