Author: Pingping Zhang; Qi Gao; Tang Wang; Yuehua Ke; Fei Mo; Ruizhong Jia; Wanbing Liu; Lei Liu; Shangen Zheng; Yuzhen Liu; Luping Li; Yao Wang; Lei Xu; Kun Hao; Ruifu Yang; Shiyue Li; Changqing Lin; Yong Zhao
Title: Evaluation of recombinant nucleocapsid and spike proteins for serological diagnosis of novel coronavirus disease 2019 (COVID-19) Document date: 2020_3_20
ID: 7atoko6c_6
Snippet: In the present study, we prepared six recombinant proteins based on the reported 16 HCoV-19 sequence (GenBank accession MN908947) [11] . Three recombinant N 17 proteins were obtained by prokaryotic expression, including the full-length 18 recombinant N protein (rN), NH 2 terminal (rN1) protein fragments, and COOH 19 terminal (rN2) fragments from this protein. Three recombinant S proteins were also 20 obtained by eukaryotic expression, including a.....
Document: In the present study, we prepared six recombinant proteins based on the reported 16 HCoV-19 sequence (GenBank accession MN908947) [11] . Three recombinant N 17 proteins were obtained by prokaryotic expression, including the full-length 18 recombinant N protein (rN), NH 2 terminal (rN1) protein fragments, and COOH 19 terminal (rN2) fragments from this protein. Three recombinant S proteins were also 20 obtained by eukaryotic expression, including an S1 domain (rS1) fragment, the 21 All rights reserved. No reuse allowed without permission. author/funder, who has granted medRxiv a license to display the preprint in perpetuity. author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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