Author: Megan C. Cohan; Ammon E. Posey; Steven J. Grigsby; Anuradha Mittal; Alex S. Holehouse; Paul J. Buske; Petra A. Levin; Rohit V. Pappu
Title: Evolved sequence features within the intrinsically disordered tail influence FtsZ assembly and bacterial cell division Document date: 2018_4_14
ID: 2rzfuy33_96
Snippet: GTPase Assay: GTP hydrolysis activity was monitored using a coupled GTPase assay (Ingerman and Nunnari, 2005) . Using a 96-well plate reader, the assay was conducted in the same buffer conditions as the light scattering assay (5 µM FtsZ WT / variant, and 1 mM GTP), and included 1 mM phosphoenolpyruvate, 250 µM NADH, and 40 units/ml of both lactose All rights reserved. No reuse allowed without permission......
Document: GTPase Assay: GTP hydrolysis activity was monitored using a coupled GTPase assay (Ingerman and Nunnari, 2005) . Using a 96-well plate reader, the assay was conducted in the same buffer conditions as the light scattering assay (5 µM FtsZ WT / variant, and 1 mM GTP), and included 1 mM phosphoenolpyruvate, 250 µM NADH, and 40 units/ml of both lactose All rights reserved. No reuse allowed without permission.
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