Author: Chan, Paul K.S.; Tam, John S.; Lam, Ching-Wan; Chan, Edward; Wu, Alan; Li, Chi-Kong; Buckley, Thomas A.; Ng, King-Cheung; Joynt, Gavin M.; Cheng, Frankie W.T.; To, Ka-Fai; Lee, Nelson; Hui, David S.C.; Cheung, Jo L.K.; Chu, Ida; Liu, Esther; Chung, Sydney S.C.; Sung, Joseph J.Y.
Title: Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome Cord-id: gafcbmlh Document date: 2003_9_25
ID: gafcbmlh
Snippet: We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidne
Document: We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.
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