Chemiluminescence assay for Listeria monocytogenes based on Cu/Co/Ni ternary nanocatalyst coupled with penicillin as generic capturing agent.

Zhang, Yue; Cui, Gaoxi; Meng, Yuchan; Wang, Yujing; Hun, Xu

Bacterial pathogens control is important in seafood production. In this study, a Cu/Co/Ni ternary nanoalloy (Cu/Co/Ni TNA) is synthesized with oleylamine reducing method. It is found that Cu/Co/Ni TNA greatly enhances the chemiluminescence (CL) signal of hydroxylamine-O-sulfonicacid (HOSA)-luminol system. The CL properties of the Cu/Co/Ni TNA are investigated systemically. And the possible CL mechanism also is intensively investigated. Based on enhanced CL phenomenon of Cu/Co/Ni TNA, a Cu/Co/Ni TNA, penicillin and anti-L. monocytogenes (Listeria monocytogenes) antibody-based sandwich complex assay for detection of L. monocytogenes is established. In this sandwich CL assay, penicillin is employed to capture and enrich pathogenic bacteria with penicillin-binding proteins (PBPs) while anti-L. monocytogenes antibody is adopted as the specific recognition molecule to recognize L. monocytogenes. L. monocytogenes is detected sensitively based on this new Cu/Co/Ni TNA-HOSA-luminol CL system. The CL intensity is proportional to the L. monocytogenes concentration ranging from 2.0 × 102 CFU mL-1 to 3.0 × 107 CFU mL-1 and the limit of detection is 70 CFU mL-1 . The reliability and potential applications of our method was verified by comparison with official method and recovery test in environment and food samples.