A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR

Mote, Ridim D; Laxmikant V, Shinde; Singh, Surya Bansi; Tiwari, Mahak; Hemant,; Srivastava, Juhi; Tripathi, Vidisha; Seshadri, Vasudevan; Majumdar, Amitabha; Subramanyam, Deepa

Real-time PCR is a widely used technique for quantification of gene expression. However, commercially available kits for real-time PCR are very expensive. The ongoing coronavirus pandemic has severely hampered the economy in a number of developing countries, resulting in a reduction in available research funding. The fallout of this will result in limiting educational institutes and small enterprises from using cutting edge biological techniques such as real-time PCR. Here, we report a cost-effective approach for preparing and assembling cDNA synthesis and real-time PCR mastermixes with similar efficiencies as commercially available kits. Our results thus demonstrate an alternative to commercially available kits.