A nematode-specific gene underlies bleomycin-response variation in Caenorhabditis elegans

Shannon C. Brady; Stefan Zdraljevic; Karol W. Bisaga; Robyn E. Tanny; Daniel E. Cook; Daehan Lee; Ye Wang; Erik C. Andersen

To test the role of natural variation in scb-1 function, we performed a reciprocal hemizygosity 622 test in control and bleomycin conditions (Figure 6, File S3) . These results matched the increase 623 in sensitivity of homozygous deletions in both parental backgrounds observed previously. The 624 hemizygous strain with the CB4856 allele of scb-1 had a bleomycin phenotype similar to the 625 heterozygous strain, whereas the hemizygous strain with the N2 allele of scb-1 was more 626 sensitive to bleomycin than the heterozygous strain. Taken together, these results suggest that 627 natural variation in scb-1 function underlies the bleomycin-response difference between the N2 628 and CB4856 strains. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/565218 doi: bioRxiv preprint