COVID-19 diagnosis by RT-qPCR in alternative specimens

Gonçalves, Cássia Cristina Alves; Barroso, Shana Priscila Coutinho; Herlinger, Alice Laschuk; Galliez, Rafael de Mello; de Almeida, Tailah Bernardo; Boullosa, Lidia Theodoro; Nascimento, Erica Ramos dos Santos; de Almeida, Jessica M; da Costa, Raissa Mirella dos Santos Cunha; da Paixão, Tatiana Monteiro; Couceiro, José Nelson dos Santos Silva; Frauches, Thiago Silva; de Souza Jr, Wilson Rodrigues; Costa, Andréa Ribeiro; Faffe, Débora Souza; Leitão, Isabela de Carvalho; da Silva, Bianca Ortiz; de Lira, Guilherme Sant’Anna; de Almeida, Isabela Labarba Carvalho; Ferreira, Orlando da Costa; Castiñeiras, Terezinha Marta Pereira Pinto; Mariani, Diana; Tanuri, Amilcar

BACKGROUND: The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent contamination risks for healthcare workers during nasopharyngeal swab (NP) sample collection and the discomfort it causes patients, brought the need to identify alternative specimens suitable for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). OBJECTIVES: The aim of this work was to compare saliva and gingival fluid swabs to NP swabs as specimens for RT-qPCR-based SARS-CoV-2 diagnosis. METHODS: We compared gingival fluid swabs (n = 158) and saliva (n = 207) to the rayon-tipped NP swabs obtained from mild-symptomatic and asymptomatic subjects as specimens for RT-qPCR for SARS-CoV-2 detection. FINDINGS: When compared to NP swabs, gingival fluid swabs had a concordance rate of 15.4% among positive samples, zero among inconclusive, and 100% among negative ones. For saliva samples, the concordance rate was 67.6% among positive samples, 42.9% among inconclusive, and 96.8% among negative ones. However, the concordance rate between saliva and NP swabs was higher (96.9%) within samples with lower cycle threshold (Ct) values (Ct > 10 ≤ 25). MAIN CONCLUSIONS: Our data suggests that whereas gingival fluid swabs are not substitutes for NP swabs, saliva might be considered whenever NP swabs are not available or recommended.