Selected article for: "ac expression and wt cell"
Author: Karen N. Barnard; Brian R. Wasik; Justin R. LaClair; Wendy S. Weichert; Brynn K. Lawrence; Colin R. Parrish
Title: Expression of 9-O- and 7,9-O-acetyl modified sialic acid in cells and their effects on influenza viruses
  • Document date: 2019_5_25
    • ID: edslp6l5_10
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/650341 doi: bioRxiv preprint heterogeneity was still seen in these populations for 9-O-Ac. This suggests that 7,9-O-227 and 9-O-Ac expression is not directly regulated by the levels of CasD1 gene expression, 228 but may be affected by post-translational regulation of CasD1 activity or removal of the 229 modifications by SIAE or oth.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/650341 doi: bioRxiv preprint heterogeneity was still seen in these populations for 9-O-Ac. This suggests that 7,9-O-227 and 9-O-Ac expression is not directly regulated by the levels of CasD1 gene expression, 228 but may be affected by post-translational regulation of CasD1 activity or removal of the 229 modifications by SIAE or other enzymes. In addition, these modifications are not 230 regulated the same across individual cells as evidenced by the population 231 heterogeneity. SIAE transcripts were seen to be consistently expressed in HEK-293 232 WT, ΔCasD1, and CasD1-OX cell populations by qPCR analysis, although SIAE mRNA 233 levels in CasD1-OX cells were lower than those seen in WT (Fig. 4E) . 234

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