Author: Karen N. Barnard; Brian R. Wasik; Justin R. LaClair; Wendy S. Weichert; Brynn K. Lawrence; Colin R. Parrish
Title: Expression of 9-O- and 7,9-O-acetyl modified sialic acid in cells and their effects on influenza viruses Document date: 2019_5_25
ID: edslp6l5_12
Snippet: To determine the role of 235 SIAE activity in regulating 7,9-O-and 9-O-Ac display, SIAE knockout (ΔSIAE) cells were 236 generated from WT HEK-293 and A549 cells by targeting early exons in the SIAE gene 237 (Fig. 5A) . Complete knockout of SIAE was confirmed both by genotyping to show 238 deletions in both alleles and by loss of mRNA by qPCR (Fig. 5A, D) . ΔSIAE cells 239.....
Document: To determine the role of 235 SIAE activity in regulating 7,9-O-and 9-O-Ac display, SIAE knockout (ΔSIAE) cells were 236 generated from WT HEK-293 and A549 cells by targeting early exons in the SIAE gene 237 (Fig. 5A) . Complete knockout of SIAE was confirmed both by genotyping to show 238 deletions in both alleles and by loss of mRNA by qPCR (Fig. 5A, D) . ΔSIAE cells 239
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