Selected article for: "blue exclusion and Cell viability"
Author: Ali Gibran; Runzhen Zhao; Mo Zhang; Krishan G. Jain; Jianjun Chang; Satoshi Komatsu; Xiaohui Fang; Beiyun Zhou; Jiurong Liang; Dianhua Jiang; Mistuo Ikebe; Michael A Matthay; Hong-Long Ji
Title: Fibrinolytic niche is requested for alveolar type 2 cell-mediated alveologenesis and injury repair
  • Document date: 2020_3_25
    • ID: 4h930ksd_56
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.006270 doi: bioRxiv preprint Human epithelial AT2 cells were isolated from six human lungs not used for transplantation by the Northern California Transplant Donor Network as previously described3 , 4. Three of the lungs were normal and three of the lungs met ARDS criteria before they were harvested. After cold preservation at 4°C, th.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.006270 doi: bioRxiv preprint Human epithelial AT2 cells were isolated from six human lungs not used for transplantation by the Northern California Transplant Donor Network as previously described3 , 4. Three of the lungs were normal and three of the lungs met ARDS criteria before they were harvested. After cold preservation at 4°C, the right middle lobe was selected for cell isolation if no apparent signs of consolidation or hemorrhage by gross inspection were seen. The cell suspension was further incubated on Petri dishes coated with human IgG antibodies (Sigma) overnight at 37°C to remove the remaining macrophages and fibroblasts. Cell viability was assessed by the trypan blue exclusion method. AT2 cell purity was evaluated by Papanicolaou (Pap) staining.

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