Author: Ali Gibran; Runzhen Zhao; Mo Zhang; Krishan G. Jain; Jianjun Chang; Satoshi Komatsu; Xiaohui Fang; Beiyun Zhou; Jiurong Liang; Dianhua Jiang; Mistuo Ikebe; Michael A Matthay; Hong-Long Ji
Title: Fibrinolytic niche is requested for alveolar type 2 cell-mediated alveologenesis and injury repair Document date: 2020_3_25
ID: 4h930ksd_73
Snippet: For analysis of AT2 cell proliferation and differentiation, AT2 organoids from different experimental groups were isolated from matrigel with dispase (10 U/mL) and dissociated in 0.25% trypsin-EDTA to get a single-cell suspension. Cells were then stained with antibodies AF488 conjugated EpCAM, APC conjugated ICAM, and APC conjugated PDPN. Gates for both colors were set by unstained cells and isotype controls for each antibody. author/funder. All .....
Document: For analysis of AT2 cell proliferation and differentiation, AT2 organoids from different experimental groups were isolated from matrigel with dispase (10 U/mL) and dissociated in 0.25% trypsin-EDTA to get a single-cell suspension. Cells were then stained with antibodies AF488 conjugated EpCAM, APC conjugated ICAM, and APC conjugated PDPN. Gates for both colors were set by unstained cells and isotype controls for each antibody. author/funder. All rights reserved. No reuse allowed without permission.
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