Selected article for: "ac internal display surface and cell line"
Author: Karen N. Barnard; Brian R. Wasik; Justin R. LaClair; Wendy S. Weichert; Brynn K. Lawrence; Colin R. Parrish
Title: Expression of 9-O- and 7,9-O-acetyl modified sialic acid in cells and their effects on influenza viruses
  • Document date: 2019_5_25
    • ID: edslp6l5_13
    Snippet: showed a small but significant increase in surface display and a large increase in 240 internal display of 9-O-Ac based on flow cytometry, but did not appear to show any 241 changes in surface or internal display of 7,9-O-Ac (Fig. 5B, C) . HPLC analysis also 242 showed a small increase of 9-O-Ac in HEK-293 cells, but no 7,9-O-Ac was detected in 243 either cell line (Fig. 5E) . When the overexpression CasD1 plasmid was transfected into 244 ΔSIAE .....
    Document: showed a small but significant increase in surface display and a large increase in 240 internal display of 9-O-Ac based on flow cytometry, but did not appear to show any 241 changes in surface or internal display of 7,9-O-Ac (Fig. 5B, C) . HPLC analysis also 242 showed a small increase of 9-O-Ac in HEK-293 cells, but no 7,9-O-Ac was detected in 243 either cell line (Fig. 5E) . When the overexpression CasD1 plasmid was transfected into 244 ΔSIAE cells (ΔSIAE+CasD1), cells showed an increase in surface and internal 9-O-Ac, 245 but no increase in surface or internal display of 7,9-O-Ac by either flow cytometry or 246 immunofluorescence microscopy (Fig. 5B,C) . HPLC confirmed the flow cytometry 247 results by showing a small increase of 9-O-Ac levels in HEK-293 cells that were similar 248 to those seen in CasD1-OX (Figs. 5E) . A549 cells showed a small but non-significant 249 All rights reserved. No reuse allowed without permission.

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