Extensive genetic diversity of bat-borne polyomaviruses reveals inter-family host-switching events

Zhizhou Tan; Gabriel Gonzalez; Jinliang Sheng; Jianmin Wu; Fuqiang Zhang; Lin Xu; Peisheng Zhang; Aiwei Zhu; Yonggang Qu; Changchun Tu; Michael J. Carr; Biao He

To investigate further the factors potentially influencing the divergence between PyVs with 286 high nt sequence identities, Mantel's correlation tests were performed to compare the divergence 287 among PyV genome sequences with those among cytb genes of their corresponding hosts and to 288 control the putative effects of the geographic location on the bat collection. As shown in Table 3 , 289 correlation analysis was performed using three groups of data: 1) a total of 42 PyVs with full 290 genomes from the present study; 2) a total of 48 PyVs including the 42 PyV genomes described The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/627158 doi: bioRxiv preprint 300 < 2×10 -4 ) and 0.65 (P < 1.5×10 -3 ), respectively. This first dataset supported the intra-host The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/627158 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/627158 doi: bioRxiv preprint 354 solution also suggested that the lineage of Rhinolophus affinis polyomavirus 2 had given rise to 355 Aselliscus stoliczkanus polyomavirus 1. Other host-switching events inferred earlier in the 356 divergence among PyVs were more difficult to corroborate with the available tMRCA data.