Author: Julia Alcoba-Florez; Rafaela Gonzalez-Montelongo; Antonio Inigo-Campos; Diego Garcia-Martinez de Artola; Helena Gil-Campesino; Laura Ciuffreda; Agustin Valenzuela-Fernandez; Carlos Flores
Title: Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples Document date: 2020_4_11
ID: icpwfyss_7
Snippet: Alternative sample treatments: Fresh FAE and RNAsnap TM buffers were prepared from Molecular Biology grade reagents following the described conditions (Stead et al., 2012; Shedlovskiy, Shcherbik and Pestov, 2017) . Aliquots of 100 μL from each VTM sample were mixed with 10 μL of each buffer using a vigorous vortexing and heated at 70 o C for 10 min. Note that the RNAsnap TM procedure was originally described to be used at 95 o C for 7 min. In p.....
Document: Alternative sample treatments: Fresh FAE and RNAsnap TM buffers were prepared from Molecular Biology grade reagents following the described conditions (Stead et al., 2012; Shedlovskiy, Shcherbik and Pestov, 2017) . Aliquots of 100 μL from each VTM sample were mixed with 10 μL of each buffer using a vigorous vortexing and heated at 70 o C for 10 min. Note that the RNAsnap TM procedure was originally described to be used at 95 o C for 7 min. In parallel, a 10 μL aliquot of each sample was also heated at 70 o C for 10 min. RT-qPCR was performed with 3.5 μL temperature-treated sample aliquots from each treatment in a 10 μL final volume using the same conditions as described for the standard diagnosis of COVID-19/SARS-CoV-2 infection.
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