Comprehensive phenotyping of endothelial cells using flow cytometry 2: Human.

Grant, Dillon; Wanner, Nicholas; Frimel, Matthew; Erzurum, Serpil; Asosingh, Kewal

In vascular research, clinical samples and samples from animal models are often used together to foster translation of preclinical findings to humans. General concepts of endothelia and murine-specific endothelial phenotypes were discussed in part 1 of this two part series. Here, in part 2, we present a comprehensive overview of human-specific endothelial phenotypes. Pan-endothelial cell markers, organ specific endothelial antigens, and flow cytometric immunophenotyping of blood-borne endothelial cells are reviewed.