Development of SPR biosensor for simultaneous detection of multiplex respiratory viruses.

Shi, Lei; Sun, Qiuxiang; He, Jian'an; Xu, Hua; Liu, Chunxiao; Zhao, Chunzhong; Xu, Yunqing; Wu, Changlin; Xiang, Junjian; Gu, Dayong; Long, Jun; Lan, Hekui

A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza A and influenza B, H1N1, respiratory syncytial virus (RSV), parainfluenza virus 1-3 (PIV1, 2, 3), adenovirus, and severe acute respiratory syndrome coronavirus (SARS). The SPR biosensor was developed by immobilizing nine respiratory virus-specific oligonucleotides in an SPR chip. To increase the biosensor sensitivity, biotin was used to label the PCR primer and further amplify the signal by introducing streptavidin after hybridization. Throat swab specimens representing nine common respiratory viruses were tested by the innovative SPR-based biosensor to evaluate the sensitivity, specificity and reproducibility of this method. Results suggest that this biosensor has the potential to simultaneously identify common respiratory viruses.