Author: Stuart Weston; Rob Haupt; James Logue; Krystal Matthews; Matthew B. Frieman
Title: FDA approved drugs with broad anti-coronaviral activity inhibit SARS-CoV-2 in vitro Document date: 2020_3_27
ID: jbc74lcu_13
Snippet: To further analyse candidate drugs, Vero cells were plated in 24 well plate format one day prior to infection. As with the drug screens, cells were pre-treated with drug at a range of concentrations, or vehicle control for 2 h. Cells were then infected with SARS-CoV-2 at MOI 0.1 for 24 h. Supernatant was collected, centrifuged in a table top centrifuge for 3 min at max speed and stored at -80°C. After a wash in PBS, infected cells were collected.....
Document: To further analyse candidate drugs, Vero cells were plated in 24 well plate format one day prior to infection. As with the drug screens, cells were pre-treated with drug at a range of concentrations, or vehicle control for 2 h. Cells were then infected with SARS-CoV-2 at MOI 0.1 for 24 h. Supernatant was collected, centrifuged in a table top centrifuge for 3 min at max speed and stored at -80°C. After a wash in PBS, infected cells were collected in TRIzol (Ambion) for RNA analysis (described below). Supernatant was used to titer viral production by TCID50 assay (6) . The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.25.008482 doi: bioRxiv preprint TaqMan Fast Advanced Master Mix. Fold change between drug treated and vehicle control was determined by calculating DDCT after normalization to the endogenous control of 18S.
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