Author: Ali Gibran; Runzhen Zhao; Mo Zhang; Krishan G. Jain; Jianjun Chang; Satoshi Komatsu; Xiaohui Fang; Beiyun Zhou; Jiurong Liang; Dianhua Jiang; Mistuo Ikebe; Michael A Matthay; Hong-Long Ji
Title: Fibrinolytic niche is requested for alveolar type 2 cell-mediated alveologenesis and injury repair Document date: 2020_3_25
ID: 4h930ksd_58
Snippet: Freshly isolated cells were seeded on either collagen IV (for mouse AT2, 10 μg/cm 2 ) or collagen I (for human AT2, 10 μg/cm 2 ) coated plates for 24 -36 h to revive CD44 expression diminished by digestive enzymes. Both unattached and attached (trypsinized) cells were collected and blocked with 1% BSA, 4% normal goat serum in PBS. Cells were stained with AF488-EpCAM (BioLegend), APC anti-human CD44 (BioLegend), and their respective isotypes. Ce.....
Document: Freshly isolated cells were seeded on either collagen IV (for mouse AT2, 10 μg/cm 2 ) or collagen I (for human AT2, 10 μg/cm 2 ) coated plates for 24 -36 h to revive CD44 expression diminished by digestive enzymes. Both unattached and attached (trypsinized) cells were collected and blocked with 1% BSA, 4% normal goat serum in PBS. Cells were stained with AF488-EpCAM (BioLegend), APC anti-human CD44 (BioLegend), and their respective isotypes. Cells were sorted using a Beckman Coulter MoFlo high-speed cell sorter. Unstained, isotype author/funder. All rights reserved. No reuse allowed without permission.
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