Author: Ali Gibran; Runzhen Zhao; Mo Zhang; Krishan G. Jain; Jianjun Chang; Satoshi Komatsu; Xiaohui Fang; Beiyun Zhou; Jiurong Liang; Dianhua Jiang; Mistuo Ikebe; Michael A Matthay; Hong-Long Ji
Title: Fibrinolytic niche is requested for alveolar type 2 cell-mediated alveologenesis and injury repair Document date: 2020_3_25
ID: 4h930ksd_66
Snippet: Anti-pdpn and anti-sftpc antibodies were used to detect AT1 and AT2 cells, respectively. Fluorescence conjugated secondary antibodies, goat anti-hamster AF568 and goat anti-rabbit IgG AF488 were used. Fluorescent images were projected with a Zeiss LSM 510 confocal microscope and stacked with a Fiji plug-in for ImageJ. Monolayers and organoids were scanned for Z sections with optimal depth from top to bottom. Images were stacked for pdpn and sftpc.....
Document: Anti-pdpn and anti-sftpc antibodies were used to detect AT1 and AT2 cells, respectively. Fluorescence conjugated secondary antibodies, goat anti-hamster AF568 and goat anti-rabbit IgG AF488 were used. Fluorescent images were projected with a Zeiss LSM 510 confocal microscope and stacked with a Fiji plug-in for ImageJ. Monolayers and organoids were scanned for Z sections with optimal depth from top to bottom. Images were stacked for pdpn and sftpc signal separately to count the number of positive cells precisely with a cell counter plug-in of ImageJ. author/funder. All rights reserved. No reuse allowed without permission.
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