Author: Said Mougari; Nisrine Chelkha; Dehia Sahmi-Bounsiar; Fabrizio Di Pinto; Philippe Colson; Jonatas Abrahao; Bernard La Scola
Title: First evidence of host range expansion in virophages and its potential impact on giant viruses and host cells Document date: 2019_9_24
ID: itxrhjns_45
Snippet: In our case, the deletion affected a part of a gene. ORF 8 in Guarani is 933 base pairs in length and contains 310 amino acids 24 . The same gene was described in all Sputnik strains (with 100% amino acid identity) and was predicted to be involved in protein-protein interactions with giant viruses within factories 6, 11 . This gene shows a remarkable repetitive pattern. Five repeats of 27 nucleotides could be detected. The deletion affects 4 repe.....
Document: In our case, the deletion affected a part of a gene. ORF 8 in Guarani is 933 base pairs in length and contains 310 amino acids 24 . The same gene was described in all Sputnik strains (with 100% amino acid identity) and was predicted to be involved in protein-protein interactions with giant viruses within factories 6, 11 . This gene shows a remarkable repetitive pattern. Five repeats of 27 nucleotides could be detected. The deletion affects 4 repeats, of which 2 repeats are completely lost (Fig. 3a ). Our PCR system targeting the deletion site confirmed the presence of a new subpopulation in Sputnik that shows the same deletion as Guarani even before contact with Tupanvirus. This finding probably explains the capacity of Sputnik to replicate with this virus in primo coculture (Supplementary Fig. 4) .
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