Author: Julia Alcoba-Florez; Rafaela Gonzalez-Montelongo; Antonio Inigo-Campos; Diego Garcia-Martinez de Artola; Helena Gil-Campesino; Laura Ciuffreda; Agustin Valenzuela-Fernandez; Carlos Flores
Title: Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples Document date: 2020_4_11
ID: icpwfyss_1
Snippet: The ongoing coronavirus disease 2019 (COVID-19) pandemic due to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) worldwide infection (https://www.who.int/emergencies/diseases/novelcoronavirus-2019/situation-reports) has imposed an unexpected high burden on the health care systems worldwide leading to an increasing demand for daily diagnostic screening. The current standard assay for diagnosis is based on the extraction of RNA from res.....
Document: The ongoing coronavirus disease 2019 (COVID-19) pandemic due to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) worldwide infection (https://www.who.int/emergencies/diseases/novelcoronavirus-2019/situation-reports) has imposed an unexpected high burden on the health care systems worldwide leading to an increasing demand for daily diagnostic screening. The current standard assay for diagnosis is based on the extraction of RNA from respiratory samples, especially from nasopharyngeal swab viral transport media (VTM), and subsequent one-step reverse transcription and real-time quantitative PCR (RT-qPCR) targeting one or several sequences from SARS-CoV-2 (Corman et al., 2020) . However, this standard procedure usually takes 3.5-4.0 h considering the manual interventions and there is a risk of reagent shortage in major kit suppliers, particularly for the RNA extraction step. Besides, the common hospital practice relies on liquid-handling robots for RNA extractions that adds another bottleneck nowadays given the large number of populations being screened.
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