Selected article for: "extraction protocol and RNA extraction protocol"
Author: Julia Alcoba-Florez; Rafaela Gonzalez-Montelongo; Antonio Inigo-Campos; Diego Garcia-Martinez de Artola; Helena Gil-Campesino; Laura Ciuffreda; Agustin Valenzuela-Fernandez; Carlos Flores
Title: Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples
  • Document date: 2020_4_11
    • ID: icpwfyss_2
    Snippet: Here we aimed to simplify the current diagnostic standard for COVID-19 by overcoming the RNA extraction step. We tested three simple approaches based on direct nasopharyngeal swab VTM heating before the RT-qPCR: a) directly without additives (Direct); b) in a formamide-EDTA (FAE) buffer (Shedlovskiy, Shcherbik and Pestov, 2017) ; and c) in a RNAsnap TM buffer (Stead et al., 2012) . As a reference, we compared their results with those obtained usi.....
    Document: Here we aimed to simplify the current diagnostic standard for COVID-19 by overcoming the RNA extraction step. We tested three simple approaches based on direct nasopharyngeal swab VTM heating before the RT-qPCR: a) directly without additives (Direct); b) in a formamide-EDTA (FAE) buffer (Shedlovskiy, Shcherbik and Pestov, 2017) ; and c) in a RNAsnap TM buffer (Stead et al., 2012) . As a reference, we compared their results with those obtained using a standard RNA extraction protocol on the same samples.

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