SARS-CoV-2 infection and transmission in school settings during the second COVID-19 wave: a cross-sectional study, Berlin, Germany, November 2020

Theuring, Stefanie; Thielecke, Marlene; van Loon, Welmoed; Hommes, Franziska; Hülso, Claudia; von der Haar, Annkathrin; Körner, Jennifer; Schmidt, Michael; Böhringer, Falko; Mall, Marcus A; Rosen, Alexander; von Kalle, Christof; Kirchberger, Valerie; Kurth, Tobias; Seybold, Joachim; Mockenhaupt, Frank P; Bozkurt, Esna; Chylla, Tanja; Bothmann, Melanie; Demirtas, Esra; Gülec, llay; Haack, Verena; Haniel, Franziska; Horn, Philipp; Kindzierski, Sophia; Kollatzsch, Mandy; Kurzmann, Marco; Lieber, Sascha; Linzbach, Elisabeth; Peters, Frederike; Rössig, Heike; Santos de Oliveira, Rafael; Steger, Julia; Tuncer, Zümrüt; Voelskow, Vanessa; Wiesmann, Christof

BACKGROUND: School attendance during the COVID-19 pandemic is intensely debated. AIM: In November 2020, we assessed SARS-CoV-2 infections and seroreactivity in 24 randomly selected school classes and connected households in Berlin, Germany. METHODS: We collected oro-nasopharyngeal swabs and blood samples, examining SARS-CoV-2 infection and IgG antibodies by RT-PCR and ELISA. Household members self-swabbed. We assessed individual and institutional prevention measures. Classes with SARS-CoV-2 infection and connected households were retested after 1 week. RESULTS: We examined 1,119 participants, including 177 primary and 175 secondary school students, 142 staff and 625 household members. SARS-CoV-2 infection occurred in eight classes, affecting each 1–2 individuals. Infection prevalence was 2.7% (95% confidence interval (CI): 1.2–5.0; 9/338), 1.4% (95% CI: 0.2–5.1; 2/140), and 2.3% (95% CI: 1.3–3.8; 14/611) among students, staff and household members. Six of nine infected students were asymptomatic at testing. We detected IgG antibodies in 2.0% (95%CI: 0.8–4.1; 7/347), 1.4% (95% CI: 0.2–5.0; 2/141) and 1.4% (95% CI: 0.6–2.7; 8/576). Prevalence increased with inconsistent facemask-use in school, walking to school, and case-contacts outside school. For three of nine households with infection(s), origin in school seemed possible. After 1 week, no school-related secondary infections appeared in affected classes; the attack rate in connected households was 1.1%. CONCLUSION: School attendance under rigorously implemented preventive measures seems reasonable. Balancing risks and benefits of school closures need to consider possible spill-over infection into households. Deeper insight is required into the infection risks due to being a schoolchild vs attending school.