Author: Nicola F Fletcher; Luke W Meredith; Emma L Tidswell; Steven R Bryden; Daniel Gonçalves-Carneiro; Yasmin Chaudhry; Claire Shannon-Lowe; Michael A Folan; Daniella A Lefteri; Marieke Pingen; Dalan Bailey; Clive S McKimmie; Alan W Baird
Title: A novel antiviral formulation inhibits a range of enveloped viruses. Document date: 2020_3_30
ID: nly9vojr_15
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.03.29.009464 doi: bioRxiv preprint Recombinant wild-type Epstein-Barr virus (EBV 2089) with a GFP insert (Delecluse et al., 1998) was generated from 293T cells carrying the recombinant EBV B95.8 genome, as previously described (Shannon-Lowe et al., 2006) . Encapsidated and enveloped virus was purified from the culture supernatants by centrifugat.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.03.29.009464 doi: bioRxiv preprint Recombinant wild-type Epstein-Barr virus (EBV 2089) with a GFP insert (Delecluse et al., 1998) was generated from 293T cells carrying the recombinant EBV B95.8 genome, as previously described (Shannon-Lowe et al., 2006) . Encapsidated and enveloped virus was purified from the culture supernatants by centrifugation on an OptiPrep (Axis Shield) self-generated gradient (Shannon-Lowe et al., 2009 ) and quantitated by quantitative PCR (qPCR) of a single-copy gene, BALF5, as previously described (Shannon-Lowe et al., 2006) .
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