A novel antiviral formulation inhibits a range of enveloped viruses.

Nicola F Fletcher; Luke W Meredith; Emma L Tidswell; Steven R Bryden; Daniel Gonçalves-Carneiro; Yasmin Chaudhry; Claire Shannon-Lowe; Michael A Folan; Daniella A Lefteri; Marieke Pingen; Dalan Bailey; Clive S McKimmie; Alan W Baird

HepG2, Caco-2, A549, Vero cells, RAW 264.7 murine macrophages, 293T human embryonic kidney cells and BV2 murine microglial cells were propagated in DMEM supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine (Gibco), antibiotics (100U of penicillin/mL and 100μg of streptomycin/mL) and 1% non-essential amino acids (Gibco). Baby hamster kidney-21 (BHK-21) cells and C6/36 mosquito cells were grown as previously described (Pingen et al., 2016) . Vero cells engineered to over-express signaling lymphocyte activation molecule (SLAM) were supplemented with 0.4mg/mL of geneticin (G418, Sigma). Human foreskin fibroblasts (HFF-1) cells were obtained from ATCC (ATCC® SCRC-1041™), and were propagated in DMEM supplemented with 15% FBS, 2mM lglutamine, antibiotics (100U of penicillin/mL and 100μg of streptomycin/mL) and 1% non-essential amino acids). Primary tonsil epithelial cells were isolated from normal tonsil tissue obtained during hypophysectomy, as previously described (Feederle et al., 2007) . Primary foetal lamb skin (FLS) cells were isolated as previously described (Scagliarini et al., 2007) and cultured in Medium 199 with 10% heat inactivated FBS.