Selected article for: "qrt PCR RNA detection and viral qrt PCR RNA detection"

Author: Banyard, Ashley C.; Johnson, N.; Voller, K.; Hicks, D.; Nunez, A.; Hartley, M.; Fooks, A. R.
Title: Repeated detection of European bat lyssavirus type 2 in dead bats found at a single roost site in the UK
  • Cord-id: hw1aijwf
  • Document date: 2009_10_20
  • ID: hw1aijwf
    Snippet: In August 2007, European bat lyssavirus type 2 (EBLV-2) was isolated from a Daubenton’s bat found at Stokesay Castle. In September 2008, another bat from the same vicinity of Stokesay Castle also tested positive for EBLV-2. This is the first occurrence of repeated detection of EBLV-2 from a single site. Here, we report the detection of low levels of viral RNA in various bat organs by qRT-PCR and detection of viral antigen by immunohistochemistry. We also report sequence data from both cases an
    Document: In August 2007, European bat lyssavirus type 2 (EBLV-2) was isolated from a Daubenton’s bat found at Stokesay Castle. In September 2008, another bat from the same vicinity of Stokesay Castle also tested positive for EBLV-2. This is the first occurrence of repeated detection of EBLV-2 from a single site. Here, we report the detection of low levels of viral RNA in various bat organs by qRT-PCR and detection of viral antigen by immunohistochemistry. We also report sequence data from both cases and compare data with those derived from other EBLV-2 isolations in the UK.

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