Author: Alcántara, Roberto; Peñaranda, Katherin; Mendoza-Rojas, Gabriel; Nakamoto, Jose A.; Dueñas, Eva; Alvarez, Daniela; Adaui, Vanessa; Milón, Pohl
Title: UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection Cord-id: ad623urt Document date: 2021_9_25
ID: ad623urt
Snippet: Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and Crispr/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach r
Document: Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and Crispr/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab.
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