Selected article for: "codon stop and orf codon stop"
Author: Mélanie Legrand; Sophie Bachellier-Bassi; Keunsook K. Lee; Yogesh Chaudhari; Hélène Tournu; Laurence Arbogast; Hélène Boyer; Murielle Chauvel; Vitor Cabral; Corinne Maufrais; Audrey Nesseir; Irena Maslanka; Emmanuelle Permal; Tristan Rossignol; Louise A. Walker; Ute Zeidler; Sadri Znaidi; Floris Schoeters; Charlotte Majgier; Renaud A. Julien; Laurence Ma; Magali Tichit; Christiane Bouchier; Patrick Van Dijck; Carol A. Munro; Christophe d’Enfert
Title: Generating genomic platforms to study Candida albicans pathogenesis
  • Document date: 2018_2_8
    • ID: 1vx62ofn_13
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/261628 doi: bioRxiv preprint nucleotides of each ORF. Gene-specific reverse primers were designed by adding the sequence 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTC-3' to the 5' end of the last 30 nucleotides of each ORF excluding the Stop codon. PCR fragments amplified from these primers are compatible for C-terminal tagging with destination vectors conta.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/261628 doi: bioRxiv preprint nucleotides of each ORF. Gene-specific reverse primers were designed by adding the sequence 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTC-3' to the 5' end of the last 30 nucleotides of each ORF excluding the Stop codon. PCR fragments amplified from these primers are compatible for C-terminal tagging with destination vectors containing the Gatewayâ„¢ cassette A. A total of 6,205 primer pairs were obtained from Invitrogen in a 96well format and are listed in Supplemental Table S1 .

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