Author: Mancuso, Patrizia; Gidaro, Antonio; Gregato, Giuliana; Raveane, Alessandro; Cremonesi, Paola; Quarna, Jessica; Caccia, Sonia; Gusso, Luca; Rusconi, Stefano; Giacomelli, Andrea; Cogliati, Chiara; Bertolini, Francesco
Title: Circulating endothelial progenitors are increased in Covidâ€19 patients and correlate with SARSâ€CoVâ€2 RNA in severe cases Cord-id: a6f8oxkm Document date: 2020_8_6
ID: a6f8oxkm
Snippet: BACKGROUND: During the course of Covidâ€19, the disease caused by the new Coronavirus SARSâ€CoVâ€2, thrombotic phenomena and/or diffuse vascular damage are frequent, and viral elements have been observed within endothelial cells. OBJECTIVES: CD146+ circulating endothelial cells (CD146+ CECs) and their progenitors (CEPs) are increased in cardiovascular, thrombotic, infectious and cancer diseases. The present study was designed to investigate their kinetics in Covidâ€19 patients. METHOD: We us
Document: BACKGROUND: During the course of Covidâ€19, the disease caused by the new Coronavirus SARSâ€CoVâ€2, thrombotic phenomena and/or diffuse vascular damage are frequent, and viral elements have been observed within endothelial cells. OBJECTIVES: CD146+ circulating endothelial cells (CD146+ CECs) and their progenitors (CEPs) are increased in cardiovascular, thrombotic, infectious and cancer diseases. The present study was designed to investigate their kinetics in Covidâ€19 patients. METHOD: We used a validated flow cytometry procedure to enumerate viable and apoptotic CD146+ CECs and CEPs in Covidâ€19 patients during the course of the disease and in patients who recovered. RESULT: Viable CEPs/mL were significantly increased in Covidâ€19 patients compared to healthy controls. This increase was observed in patients with mild symptoms and not further augmented in patients with severe symptoms. In patients who recovered, CEPs decreased, but were in a range still significantly higher than normal controls. Regarding mature CD146+ CECs, in Covidâ€19 patients their absolute number was similar to those observed in healthy controls, but the viable/apoptotic CD146+ CEC ratio was significantly different. Both mild and severe Covidâ€19 patients had significantly less apoptotic CD146+ CECs compared to healthy controls. Patients who recovered had significantly less CD146+ CECs/mL when compared to controls as well as to mild and severe Covidâ€19 patients. A positive correlation was found between the copies of SARSâ€CoVâ€2 RNA in the cellular fraction and apoptotic CEPs/mL in severe Covidâ€19 patients. CONCLUSIONS: CD146+ CECs and CEPs might be investigated as candidate biomarkers of endothelial damage in Covidâ€19 patients.
Search related documents:
Co phrase search for related documents- Try single phrases listed below for: 1
Co phrase search for related documents, hyperlinks ordered by date